Journal:

Article Title: In Silico Cloning of Novel Endothelial-Specific Genes

doi:

Figure Lengend Snippet: Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project

Article Snippet: Table shows the status of expression of the five reference endothelial-specific genes in these two SAGE pools. table ft1 table-wrap mode="anchored" t5 Table 4 caption a7 Symbol Description SAGE_HCT116 Colon, cell line derived from colorectal carcinoma SAGE_Caco_2 Colon, colorectal carcinoma cell line SAGE_Duke_H392 Brain, Duke glioblastoma multiforme cell line SAGE_SW837 Colon, cancer cell line SAGE_RKO Colon, cancer cell line SAGE_NHA(5th) Brain, normal human astrocyte cells harvested at passage 5 SAGE_ES2-1 Ovarian clear cell carcinoma cell line ES-2, poorly differentiated SAGE_OVCA432-2 Ovary, carcinoma cell line OVCA432 SAGE_OV1063-3 Ovary, carcinoma cell line OV1063 SAGE_Duke_mhh-1 Brain, c-myc negative medulloblastoma cell line mhh-1 SAGE_Duke-H341 Brain, c-myc positive medulloblastoma cell line H341 SAGE_HOSE_4 Ovary, normal surface epithelium SAGE_OVP-5 Ovary, pooled cancer cell lines SAGE_LNCaP Prostate, cell line, androgen dependent SAGE_HMEC-B41 Cell culture HMEC-B41 of normal human mammary epithelial cells SAGE_MDA453 Cell line MDA-MB-453 of human breast carcinoma SAGE_SKBR3 ATCC cell line SK-BR-3, human breast adenocarcinoma SAGE_A2780-9 Ovary, ovarian cancer cell line A2780 SAGE_Duke_H247_normal Brain, glioblastoma multiforme cell line H247 SAGE_Duke_H247_Hypoxia Brain, Duke glioblastoma multiforme cell line H247, grown under 1.5% oxygen SAGE_Duke_post_crisis_fibroblasts Skin, postcrisis survival fibroblast cell line SAGE_Duke_precrisis_fibroblasts Skin, large T antigen transformed human fibroblasts clones SAGE_A Prostate, cancer cell line, induced with synthetic androgen SAGE_IOSE29-11 Ovary, surface epithelium line Open in a separate window Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project table ft1 table-wrap mode="anchored" t5 Table 5 caption a7 Known endothelial-specific gene Tags in the nonendothelial SAGE libraries Tags in the endothelial SAGE libraries von Willebrand factor (vWF) 1 (colon carcinoma cell line) 80 FLT1 VEGF receptor — — KDR VEGF receptor 1 (IOSE29 ovarian surface epithelium cell line) 6 TIE1 tyrosine kinase 17 (ovarian tumour and normal ovarian epithelium cell lines) 27 TIE2 / TEK tyrosine kinase 4 (ovarian carcinoma and glioblastoma multiforme cell lines) 2 Open in a separate window TIE1 and TIE2 / TEK have multiple hits in the nonendothelial pool (most in normal or carcinoma cell lines of ovarian origin). vWF is most endothelial specific, having 80 hits in the endothelial pool and only one hit in the nonendothelial pool.

Techniques: Expressing, Derivative Assay, Cell Culture, Transformation Assay, Clone Assay

Journal:

Article Title: In Silico Cloning of Novel Endothelial-Specific Genes

doi:

Figure Lengend Snippet: Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project

Article Snippet: Table shows the status of expression of the five reference endothelial-specific genes in these two SAGE pools. table ft1 table-wrap mode="anchored" t5 Table 4 caption a7 Symbol Description SAGE_HCT116 Colon, cell line derived from colorectal carcinoma SAGE_Caco_2 Colon, colorectal carcinoma cell line SAGE_Duke_H392 Brain, Duke glioblastoma multiforme cell line SAGE_SW837 Colon, cancer cell line SAGE_RKO Colon, cancer cell line SAGE_NHA(5th) Brain, normal human astrocyte cells harvested at passage 5 SAGE_ES2-1 Ovarian clear cell carcinoma cell line ES-2, poorly differentiated SAGE_OVCA432-2 Ovary, carcinoma cell line OVCA432 SAGE_OV1063-3 Ovary, carcinoma cell line OV1063 SAGE_Duke_mhh-1 Brain, c-myc negative medulloblastoma cell line mhh-1 SAGE_Duke-H341 Brain, c-myc positive medulloblastoma cell line H341 SAGE_HOSE_4 Ovary, normal surface epithelium SAGE_OVP-5 Ovary, pooled cancer cell lines SAGE_LNCaP Prostate, cell line, androgen dependent SAGE_HMEC-B41 Cell culture HMEC-B41 of normal human mammary epithelial cells SAGE_MDA453 Cell line MDA-MB-453 of human breast carcinoma SAGE_SKBR3 ATCC cell line SK-BR-3, human breast adenocarcinoma SAGE_A2780-9 Ovary, ovarian cancer cell line A2780 SAGE_Duke_H247_normal Brain, glioblastoma multiforme cell line H247 SAGE_Duke_H247_Hypoxia Brain, Duke glioblastoma multiforme cell line H247, grown under 1.5% oxygen SAGE_Duke_post_crisis_fibroblasts Skin, postcrisis survival fibroblast cell line SAGE_Duke_precrisis_fibroblasts Skin, large T antigen transformed human fibroblasts clones SAGE_A Prostate, cancer cell line, induced with synthetic androgen SAGE_IOSE29-11 Ovary, surface epithelium line Open in a separate window Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project table ft1 table-wrap mode="anchored" t5 Table 5 caption a7 Known endothelial-specific gene Tags in the nonendothelial SAGE libraries Tags in the endothelial SAGE libraries von Willebrand factor (vWF) 1 (colon carcinoma cell line) 80 FLT1 VEGF receptor — — KDR VEGF receptor 1 (IOSE29 ovarian surface epithelium cell line) 6 TIE1 tyrosine kinase 17 (ovarian tumour and normal ovarian epithelium cell lines) 27 TIE2 / TEK tyrosine kinase 4 (ovarian carcinoma and glioblastoma multiforme cell lines) 2 Open in a separate window TIE1 and TIE2 / TEK have multiple hits in the nonendothelial pool (most in normal or carcinoma cell lines of ovarian origin). vWF is most endothelial specific, having 80 hits in the endothelial pool and only one hit in the nonendothelial pool.

Techniques: Expressing, Derivative Assay, Cell Culture, Transformation Assay, Clone Assay

Journal:

Article Title: In Silico Cloning of Novel Endothelial-Specific Genes

doi:

Figure Lengend Snippet: Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project

Article Snippet: Table shows the status of expression of the five reference endothelial-specific genes in these two SAGE pools. table ft1 table-wrap mode="anchored" t5 Table 4 caption a7 Symbol Description SAGE_HCT116 Colon, cell line derived from colorectal carcinoma SAGE_Caco_2 Colon, colorectal carcinoma cell line SAGE_Duke_H392 Brain, Duke glioblastoma multiforme cell line SAGE_SW837 Colon, cancer cell line SAGE_RKO Colon, cancer cell line SAGE_NHA(5th) Brain, normal human astrocyte cells harvested at passage 5 SAGE_ES2-1 Ovarian clear cell carcinoma cell line ES-2, poorly differentiated SAGE_OVCA432-2 Ovary, carcinoma cell line OVCA432 SAGE_OV1063-3 Ovary, carcinoma cell line OV1063 SAGE_Duke_mhh-1 Brain, c-myc negative medulloblastoma cell line mhh-1 SAGE_Duke-H341 Brain, c-myc positive medulloblastoma cell line H341 SAGE_HOSE_4 Ovary, normal surface epithelium SAGE_OVP-5 Ovary, pooled cancer cell lines SAGE_LNCaP Prostate, cell line, androgen dependent SAGE_HMEC-B41 Cell culture HMEC-B41 of normal human mammary epithelial cells SAGE_MDA453 Cell line MDA-MB-453 of human breast carcinoma SAGE_SKBR3 ATCC cell line SK-BR-3, human breast adenocarcinoma SAGE_A2780-9 Ovary, ovarian cancer cell line A2780 SAGE_Duke_H247_normal Brain, glioblastoma multiforme cell line H247 SAGE_Duke_H247_Hypoxia Brain, Duke glioblastoma multiforme cell line H247, grown under 1.5% oxygen SAGE_Duke_post_crisis_fibroblasts Skin, postcrisis survival fibroblast cell line SAGE_Duke_precrisis_fibroblasts Skin, large T antigen transformed human fibroblasts clones SAGE_A Prostate, cancer cell line, induced with synthetic androgen SAGE_IOSE29-11 Ovary, surface epithelium line Open in a separate window Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project table ft1 table-wrap mode="anchored" t5 Table 5 caption a7 Known endothelial-specific gene Tags in the nonendothelial SAGE libraries Tags in the endothelial SAGE libraries von Willebrand factor (vWF) 1 (colon carcinoma cell line) 80 FLT1 VEGF receptor — — KDR VEGF receptor 1 (IOSE29 ovarian surface epithelium cell line) 6 TIE1 tyrosine kinase 17 (ovarian tumour and normal ovarian epithelium cell lines) 27 TIE2 / TEK tyrosine kinase 4 (ovarian carcinoma and glioblastoma multiforme cell lines) 2 Open in a separate window TIE1 and TIE2 / TEK have multiple hits in the nonendothelial pool (most in normal or carcinoma cell lines of ovarian origin). vWF is most endothelial specific, having 80 hits in the endothelial pool and only one hit in the nonendothelial pool.

Techniques: Expressing, Derivative Assay, Cell Culture, Transformation Assay, Clone Assay

Journal:

Article Title: In Silico Cloning of Novel Endothelial-Specific Genes

doi:

Figure Lengend Snippet: Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project

Article Snippet: Table shows the status of expression of the five reference endothelial-specific genes in these two SAGE pools. table ft1 table-wrap mode="anchored" t5 Table 4 caption a7 Symbol Description SAGE_HCT116 Colon, cell line derived from colorectal carcinoma SAGE_Caco_2 Colon, colorectal carcinoma cell line SAGE_Duke_H392 Brain, Duke glioblastoma multiforme cell line SAGE_SW837 Colon, cancer cell line SAGE_RKO Colon, cancer cell line SAGE_NHA(5th) Brain, normal human astrocyte cells harvested at passage 5 SAGE_ES2-1 Ovarian clear cell carcinoma cell line ES-2, poorly differentiated SAGE_OVCA432-2 Ovary, carcinoma cell line OVCA432 SAGE_OV1063-3 Ovary, carcinoma cell line OV1063 SAGE_Duke_mhh-1 Brain, c-myc negative medulloblastoma cell line mhh-1 SAGE_Duke-H341 Brain, c-myc positive medulloblastoma cell line H341 SAGE_HOSE_4 Ovary, normal surface epithelium SAGE_OVP-5 Ovary, pooled cancer cell lines SAGE_LNCaP Prostate, cell line, androgen dependent SAGE_HMEC-B41 Cell culture HMEC-B41 of normal human mammary epithelial cells SAGE_MDA453 Cell line MDA-MB-453 of human breast carcinoma SAGE_SKBR3 ATCC cell line SK-BR-3, human breast adenocarcinoma SAGE_A2780-9 Ovary, ovarian cancer cell line A2780 SAGE_Duke_H247_normal Brain, glioblastoma multiforme cell line H247 SAGE_Duke_H247_Hypoxia Brain, Duke glioblastoma multiforme cell line H247, grown under 1.5% oxygen SAGE_Duke_post_crisis_fibroblasts Skin, postcrisis survival fibroblast cell line SAGE_Duke_precrisis_fibroblasts Skin, large T antigen transformed human fibroblasts clones SAGE_A Prostate, cancer cell line, induced with synthetic androgen SAGE_IOSE29-11 Ovary, surface epithelium line Open in a separate window Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project table ft1 table-wrap mode="anchored" t5 Table 5 caption a7 Known endothelial-specific gene Tags in the nonendothelial SAGE libraries Tags in the endothelial SAGE libraries von Willebrand factor (vWF) 1 (colon carcinoma cell line) 80 FLT1 VEGF receptor — — KDR VEGF receptor 1 (IOSE29 ovarian surface epithelium cell line) 6 TIE1 tyrosine kinase 17 (ovarian tumour and normal ovarian epithelium cell lines) 27 TIE2 / TEK tyrosine kinase 4 (ovarian carcinoma and glioblastoma multiforme cell lines) 2 Open in a separate window TIE1 and TIE2 / TEK have multiple hits in the nonendothelial pool (most in normal or carcinoma cell lines of ovarian origin). vWF is most endothelial specific, having 80 hits in the endothelial pool and only one hit in the nonendothelial pool.

Techniques: Expressing, Derivative Assay, Cell Culture, Transformation Assay, Clone Assay

Journal:

Article Title: In Silico Cloning of Novel Endothelial-Specific Genes

doi:

Figure Lengend Snippet: Experimental verification by reverse transcription polymerase chain reaction (RT-PCR). Candidate endothelial-specific genes predicted by the combination of the UniGene/EST screen and xProfiler serial analysis of gene expression (SAGE) differential analysis (Table ​(Table8)8) were checked for expression in three endothelial and nine nonendothelial cell cultures. Endothelial cultures were as follows: HMVEC (human microvascular endothelial cells), HUVEC (human umbilical vein endothelial cells) confluent culture, and HUVEC proliferating culture. Nonendothelial cultures were as follows: normal endometrial stromal (NES) cells grown in normoxia and NES grown in hypoxia, MDA 453 and MDA 468 breast carcinoma cell lines, HeLa, FEK4 fibroblasts cultured in normoxia and FEK4 fibroblasts cultured in hypoxia, SW480, and HCT116, the last two being colorectal epithelium cell lines. ECSM1 and ECSM2 showed complete endothelial specificity, whereas ECSM3 and magic roundabout were very strongly preferentially expressed in the endothelium. Interestingly, all these novel genes appear more specific than the benchmark endothelial-specific gene, von Willebrand factor.

Article Snippet: Table shows the status of expression of the five reference endothelial-specific genes in these two SAGE pools. table ft1 table-wrap mode="anchored" t5 Table 4 caption a7 Symbol Description SAGE_HCT116 Colon, cell line derived from colorectal carcinoma SAGE_Caco_2 Colon, colorectal carcinoma cell line SAGE_Duke_H392 Brain, Duke glioblastoma multiforme cell line SAGE_SW837 Colon, cancer cell line SAGE_RKO Colon, cancer cell line SAGE_NHA(5th) Brain, normal human astrocyte cells harvested at passage 5 SAGE_ES2-1 Ovarian clear cell carcinoma cell line ES-2, poorly differentiated SAGE_OVCA432-2 Ovary, carcinoma cell line OVCA432 SAGE_OV1063-3 Ovary, carcinoma cell line OV1063 SAGE_Duke_mhh-1 Brain, c-myc negative medulloblastoma cell line mhh-1 SAGE_Duke-H341 Brain, c-myc positive medulloblastoma cell line H341 SAGE_HOSE_4 Ovary, normal surface epithelium SAGE_OVP-5 Ovary, pooled cancer cell lines SAGE_LNCaP Prostate, cell line, androgen dependent SAGE_HMEC-B41 Cell culture HMEC-B41 of normal human mammary epithelial cells SAGE_MDA453 Cell line MDA-MB-453 of human breast carcinoma SAGE_SKBR3 ATCC cell line SK-BR-3, human breast adenocarcinoma SAGE_A2780-9 Ovary, ovarian cancer cell line A2780 SAGE_Duke_H247_normal Brain, glioblastoma multiforme cell line H247 SAGE_Duke_H247_Hypoxia Brain, Duke glioblastoma multiforme cell line H247, grown under 1.5% oxygen SAGE_Duke_post_crisis_fibroblasts Skin, postcrisis survival fibroblast cell line SAGE_Duke_precrisis_fibroblasts Skin, large T antigen transformed human fibroblasts clones SAGE_A Prostate, cancer cell line, induced with synthetic androgen SAGE_IOSE29-11 Ovary, surface epithelium line Open in a separate window Twenty-four Nonendothelial Cell Serial Analysis Gene Expression (SAGE)–Cancer Genome Anatomy Project table ft1 table-wrap mode="anchored" t5 Table 5 caption a7 Known endothelial-specific gene Tags in the nonendothelial SAGE libraries Tags in the endothelial SAGE libraries von Willebrand factor (vWF) 1 (colon carcinoma cell line) 80 FLT1 VEGF receptor — — KDR VEGF receptor 1 (IOSE29 ovarian surface epithelium cell line) 6 TIE1 tyrosine kinase 17 (ovarian tumour and normal ovarian epithelium cell lines) 27 TIE2 / TEK tyrosine kinase 4 (ovarian carcinoma and glioblastoma multiforme cell lines) 2 Open in a separate window TIE1 and TIE2 / TEK have multiple hits in the nonendothelial pool (most in normal or carcinoma cell lines of ovarian origin). vWF is most endothelial specific, having 80 hits in the endothelial pool and only one hit in the nonendothelial pool.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture